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In the next step, PCR-amplified LPETG-MT3 was double digested with NdeI and XhoI restriction enzymes and was ligated into the same sites of the plasmid pETM11-SUMO-eSrtA-GFP to make the eSrtA-MT3 plasmid having the sequence pETM11-SUMO-eSrtA-LPETG-MT3

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