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Doxorubicin stock solution (10 mM, 100% DMSO) was serially diluted in unbuffered seahorse assay medium to prepare 6 concentrations: 400, 300, 200, 100, 80 and 40 M

132 Overall, the fibrosis-promoting and fibrosis-suppressing functions of senescence seemed to be incompatible, but in fact most senescent cells secrete SASPs that impact fibrosis development, which is a vital mechanism in OSF progression and should be comprehensively explored in the future

Astrocyte Ca 2+ hyperactivity can promote the release of detrimental factors, alter neuronal-glial communication, and impair synaptic transmission (Frost and Li, 2017

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