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Moreover, mRNA expression and activity of GCL, also displayed circadian rhythms, which were lost with loss of CYC or PER, directly linking GSH synthesis and the circadian clock (19)

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The supernatant of lysed cells was collected by centrifugation (13,000 g at 4 C for 30 min) and transferred to cleaned Ni-NTA agrose resin in 2 mL Eppendorf tubes