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Subsequently, the Fe 3+ combines to transferrin and forms a complex, which then is transported to various tissues and organs throughout the body ( 3+ and transferrin, resulting in the import of iron into cells ( 2+ , further being released to labile iron pool (LIP) ( 3+ can be reduced to Fe 2+ in the lysosome by six transmembrane epithelial antigens of prostate 3, and subsequently, zinc-iron regulatory protein family 8/14 or DMT1 leads to the release of Fe 2+ from the lysosome into LIP (40, 41)

Following extensive washing with PBST, binding of primary reagents was visualized by overnight incubation in PBST2% BSA containing donkey anti-rabbit Alexa 568 (Thermo Fisher Scientific catalog number A10042, RRID: AB_2534017) and goat anti-mouse Alexa488 (Thermo Fisher Scientific catalog number A-11070, RRID: AB_2534114)

Cell lines used in this study were RPMI-8866 cells, HEK293T cells, and Vero cells (parental and MICA-expressing)

In- vivo study suggested that, prophylactic inhibition of catecholamine synthesis by a tyrosine hydroxylase antagonist- metyrosine, reduced levels of catecholamines and cytokine responses and resulted in markedly increased various inflammatory stimuli in mice