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Green Synthesis of Silver Nanoparticles From Flower Extract of Hibiscus Rosa Sinensis and Its Antibacterial Activity. International Journal of Innovative Research in Science, Engineering and Technology 5, no

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After washing with TBS buffer containing 0.1% (v/v) Tween 20 (TBST), the samples were incubated with fluorescent secondary antibody (1:100, Cat# SA00013-2, Proteintech, China) diluted in PBS containing 0.1% (v/v) Tween 20 for 1 h at room temperature, stained with DAPI, and observed under fluorescence microscope (Zeiss Apotome)