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The supernatant containing His-tagged histones was then incubated with NiNTA resin (Complete His-Tag Purification Resin, Roche) equilibrated with buffer B (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 6 M urea, 5 mM imidazole, and 5% glycerol) for 1 h with end-over-end mixing at 4 C

In the study, purified aspalathin from a fermented rooibos extract increased dose-dependently and significantly the glucose uptake by L6 myotubes at concentrations of 1-100 M, irrespective of insulin absence and also increased insulin secretion from cultured RIN-5F cells at 100 M

SAMTOR is an S -adenosylmethionine sensor for the mTORC1 pathway

In addition, although our mechanistic gain- and loss-of-function studies were supported by extensive in vivo validation, part of the functional characterization was performed using HL60-derived neutrophil cells