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doi:10.1007/bf00280883 Chen BM, Xia LW, Zhao RQ

Immunohistochemical and immunofluorescence analyses Immunohistochemistry (IHC) staining was performed on the above jejunum or colon tissue sections by using primary antibodies of anti-MCC (1:500), anti-MCT (1:500), anti-ZO1 (1:500), anti-Claudin-2 (1:300), and anti-Occludin (1:300), and secondary antibody of HRP goat anti-rabbit IgG (1:200)

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For the setting of the heme concentration range, in pilot experiments performed previously, we compared the MALDI-TOF MS signal intensity of heme standard with the signal of the patients tissues, and roughly determined a range (data not shown)