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Intercalated cholesterol may disrupt electrostatic interactions between the ionic phosphocholine head groups of nearby membrane phospholipids, increasing their mobility, but by alignment with their acyl chains, the conformational freedom is reduced

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This process is morphologically marked by small mitochondria with condensed membrane densities, and biochemically by iron overload and GSH depletion, leading to loss of antioxidant defense and irreversible oxidative damage ( 3.2 Key molecular drivers of ferroptosis Iron Overload: Excess intracellular ferrous iron (Fe 2+ ) catalyzes the Fenton reaction, producing hydroxyl radicals (OH) that initiate lipid peroxidation cascades

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