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A gentle, consistent approach is the correct approach

Originally identified in the early 1990s, BPC-157, short for Body Protection Compound 157, also known as Gastric Pentadecapeptide, is a synthetic peptide composed of 15 amino acids and derived from a protective protein sequence found in human gastric juice

At the core of these processes lies redox balanceparticularly the role of glutathione as the bodys master antioxidant and its intimate relationship with mitochondrial health

In order to recover GST-tagged protein from the complicated mixture of proteins, the fusion protein is incubated with agarose beads coupled to glutathione (GSH-agarose, see picture below), what leads to efficient precipitation of GST-fusion protein