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[The Working Group noted that, in the absence of additional experimental results, it was impossible to determine whether ROS directly resulting from aspartame exposure led to cytotoxicity or whether toxicity caused by aspartame led to ROS generation secondary to necrotic and/or apoptotic cell death.] The impact of aspartame on membrane permeability was studied in primary glomerular microvascular endothelial cells (Enuwosa et al., 2021)