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Acta 1840, 13131330

Therapeutics and COVID-19: Living Guideline

Similarly, the use of 1-propenyl magnesium bromide as a nucleophile afforded amine 6b , and no vinyl ketone analogs were detected

This means: Cleavage leaves a completely native N-terminus no extra residues Extremely specificno off-target cleavage Efficienttypically complete cleavage in 12 hours at room temperature The cleaved SUMO tag can be removed by reverse Ni-NTA (if His6-SUMO was used) Affinity purification: Typically used as His6-SUMO, relying on the His6 tag for Ni-NTA purification