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Nitrocellulose membranes were incubated with HRP-labeled secondary antibodies (1:2,000, Santa Cruz Biotechnology, Heidelberg, Germany) according to the species of primary anti-bodies and bands were detected using Clarity Western ECL substrate (Bio-Rad, Milan, Italy)

Review Article OPEN ACCESS Anup Ramachandran Received: Revised: Accepted: Published online: Author information Citation: Jaeschke H, Ramachandran A

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Regular supplementation helps maintain healthy nerve function especially important for patients on metformin or with absorption issues