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Leave-on formulations are generally more effective than rinse-off products because they allow time for the peptide to penetrate through the outer skin layer to the dermis, where follicles are located

H 2 O 2 Measurement We measure H 2 O 2 of various concentrations in MEM medium supplemented with 10%FBS to obtain a calibration curve for H 2 O 2 detection

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Furthermore, membranes were washed with PBS-T and incubated overnight at 4 C with primary antibodies for TH at dilution of 1:1000 (Thermo Fisher Scientific, #OPA1-04050) and the sites of antibodyantigen reaction were visualized with horseradish peroxidase-conjugated secondary antibodies (P448, diluted 1:3000