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Alternatively, we also optimized the cell sorting procedure by separating the bead/cell complexes through free settling instead of using the magnetic stand, as described previously ( RNA extraction and RT-qPCR Total RNA was extracted using the GeneJET RNA Purification Kit (K0732, Thermo) according to the manufacturers instructions

In the extracellular environment, the identification of these proteins by cells of the immune system results in signal transduction that leads to the release of proinflammatory cytokines, such as IFN

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