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GPX4 knockdown GPX4 was suppressed in metM-Wnt lung cells by lentiviral transduction for 48 h, using particles generated by transfection of HEK293T cells with psPAX2, pMD2.G, and GPX4-targeting shRNAs in a psi-LVRU6H vector (Genecopoeia, GCACGAATTCTCAGCCAAGGA [shGPX4-A], GCTGGGAAATGCCATCAAATG [shGPX4-C], and GCTTCGCGCCGTAGTCTTA [Scram]) and were selected for using hygromycin (500 g/ml)