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Culture conditions Slices were maintained in incubators at 37 C, under a humidified atmosphere containing 5% CO2 in six-well plates containing 1 ml of slice culture medium, containing basal Earles salts medium (BME, sigma, MFCD00217343), 25% Hanks balanced salt solution (Sigma, H9394), 27 mM glucose (Sigma, G8769), 1% penicillin/streptomycin (Life technology, 15140122), 1 mM glutamine (Sigma, 228034), and 5% horse serum (New Zealand origin, heat-inactivated

What to Expect Prior to your first appointment, please make a list of any over-the-counter or prescription medications you're currently taking, as some medications can have an impact on weight

But some pets, especially those with gastrointestinal disease, may not be able to absorb B12 effectively