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Regular monitoring should include assessment of glycemic control in diabetic patients, renal function, and evaluation for adverse effects

The secondary outcomes will be the serum vitamin B12 concentration (pg/ml), adverse events (description, moment of onset and resolution, intensity, cause, steps taken), adherence to treatment (measured at each patient visit via the number of vials used for patients in the oral arm, and the number of injections given in the intramuscular arm), quality of life (measured using the EuroQoL-5D questionnaire), and patient satisfaction and preferences

The assay mix, containing Assay Loading Reagent (Fluidigm), DNA Suspension Buffer (Teknova), and combined forward and reverse primers, was loaded into the assay inlets of the IFC