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At that point the nerve is close to the surface with very little padding to protect it

Expression profile of the GSTM group using real-time PCR A two-step qRT-PCR approach was considered using SYBR-Green I (Brilliant SYBR Green QPCR master Mix, Agilent Technologies, CA, USA)

Ok, so these are interesting points but please add this into the mix for future answers: The NICE Guideline clearly states that there is insufficient research why some people need more frequent injections than others and that an 8- or 12- weekly schedule does not work for everyone and some may need more frequently