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In a study, human umbilical vein endothelial cells were exposed to TSA or had HDAC6 levels reduced, resulting in a reduction of vascular endothelial cell injury caused by UA and an increase in fibroblast growth factor (FGF)21 expression, as well as the activation of AKT, endothelial nitric oxide synthase (eNOS) and forkhead box (Fox)O3a

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To assess the number of redox-sensitive Cys residues in GSAAT, the enzyme was treated with hydrogen peroxide or DTT (0.110 mM DTT) and subsequently with N-ethylmaleimide (NEM) to irreversibly block the free thiol groups of Cys residues in GSAAT