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A: Cyanocobalamin (vitamin B12) and glycine are added to support side effect management and muscle preservation

Peptide mapping by mass spectrometry To probe the binding sites of MeCbl on GSDME, 0.4 M recombinant human GSDME (CSB-EP006766HU) purchased from CUSABIO (Wuhan, China) was incubated with 10 M MeCbl in 100 L buffer (20 mM HEPES, 150 mM NaCl and 50 M DPA) at room temperature for 2 h and protected from light

Per standard laboratory protocols and manufacturer recommendations (such as those from Hospira), bacteriostatic water should be discarded 28 days after the first puncture

The threshold itself may be set too low