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Longer-term follow-up and a larger patient cohort are needed to assess the sustainability of these outcomes and the cost-effectiveness of this innovative care model to support its expansion and increased capacity

qRT-PCR was performed in QuantStudio 3 realtime PCR system (Applied Biosystems, USA) using ChamQ SYBR qPCR Master Mix (Low ROX Premixed) (Q331-02, Vazyme)

Moreover, L-AST treatment resulted in decreased STAT3 and NF-B transcriptional activity in PA-induced skin tissues, suggesting its potential benefits over free AST for AD treatment [117]