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Results from a commercial kit (purchased from Roche) measuring cell survival by detecting leakage of lactate dehydrogenase (LDH) were found to be misleading for this cell model Comparison of results obtained from the two different methods showed that the LDH detection did not correlate with the NR assay for all inducers tested and was not correlated with obvious morphological changes observed by light microscopy

Notably, after TRIM21 knockdown, the ubiquitination level of SLC3A2 was significantly reduced, suggesting that TRIM21 may act as a ubiquitination enzyme for SLC3A2 (Fig