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It is a small and soluble protein with four conserved Cys residues capable of ligating one 2Fe-2S cluster (Hanke and Mulo, + reductase, for the reduction of NADP + to NADPH (Hase et al., One example of NEET-type 2Fe-2S proteins is At-NEET, a CDGSH motif-containing Arabidopsis thaliana protein dually targeted to the chloroplast stroma and mitochondria (Nechushtai et al., 2012

Avoid Chelators EDTA and other chelating agents will strip the copper ion from the peptide complex, rendering it inactive

Yang J, Tong Q, Zhang Y, Yuan S, Gao Y, Deng K, Wang Y, Lu J, Xie X, Zhang Z (2021) Overexpression of nicotinamide N -methyltransferase mainly covers stroma of colorectal cancer and correlates with unfavorable survival by its product 1-MNA

This indicates its promise as a viable treatment target for addressing tissue damage linked to hemochromatosis (Wang et al., 2017)