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Results Deletion of PRX3 is tumor suppressive To investigate the functional role of PRX3 expression in supporting mesothelioma growth we established CRISPR/Cas9 knockout of PRX3 in the human biphasic mesothelioma cell line H-MESO-1, generating two clones, H-Meso PRX3-1 , a compound heterozygote deletion, and H-Meso PRX3-2 , a homozygote deletion (referred to as PRX3 KO clones) (Fig