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After 30 min, a 100 L of acidic stop solution was added into each well and the absorbance at 450 nm was recorded using a using a Biotek Synergy Neo2 HTS Multi-Mode Microplate Reader (Mnnedorf, Switzerland)

Of the selenoproteins, SARS-CoV-2 significantly suppressed the mRNA expression of GPX4, TXNRD3 and ER-resident SELENOF, SELENOK, SELENOM and SELENOS (Reference Wang, Huang and Sun66)