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Materials and Methods Hydrogel Substrate Preparation and Optical Setup for H 2 O 2 Detection In order to perform time-lapse detection of H 2 O 2 secreted from living cells cultured in different formats, a hydrogel-based substrate capable of generating optical spectrum shift while reacting with H 2 O 2 due to LSPR and enzyme immunoprecipitation is exploited in the experiments as shown in Figure 1A

For RNA-seq analysis of amdh-1(uth29) mutants, the raw sequencing data were uploaded to the Galaxy project web platform and the public server at usegalaxy.org was used to analyze the data (Afgan et al., 2016)

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