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To stain phospho-(T94)Tctex-1, RPE-1 cells were first incubated with 0.1% Triton X-100supplemented PHEM buffer (100 mM PIPES, 100 mM Hepes, 2 mM MgSO 4 , 0.2 mM EDTA, and 4 mM EGTA, pH 6.9) for 30 s at room temperature before 4% PFA fixation for 10 min

Most importantly, vonoprazan-based dual and triple therapies consistently demonstrate high eradication rates, rivaling or surpassing BQT with fewer adverse effects even in areas of high levofloxacin and clarithromycin resistance (Tungtrongchitr et al., 2024)