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Moreover, we and others demonstrated that in addition to BACE1, the SCF-Fbx2-E3 ligase is involved in binding and ubiquitination of the NMDA and AMPA receptors at the synaptic membrane, affecting receptor turnover during neuronal activation (Kato et al., 2005

IGF-1 is the downstream protein that mediates the effects of growth hormone

Cells were stained for 30 min on ice, washed twice in FACS buffer, fixed in 200 l of 10% formalin 15 min on ice, washed and resuspended in 200 l FACS buffer for acquisition and analyzed on a BDFACS Celesta