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Gene 104(2): 147-153, 1991

PVDF membranes were blocked in 5% (w/v) milk powder in PBST (140 mM NaCl, 2.7 mM KCl, 10.1 mM Na 2 HPO4, 1.8 mM KH 2 PO4, 0.1% (v/v) Tween-20) or TBST (150 mM NaCl, 20 mM Tris/HCl, pH 7.5, 0.1 % (v/v) Tween-20) for 1 h at room temperature

For this purpose, the allicin sample was dissolved in methanol-D4, and the 1H spectrum was recorded at 298 K using a Bruker Avance III 500 spectrometer

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