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Additionally, the site equivalent to the most widespread cystic fibrosis linked mutant, (CFTR delF506) Ycf1 F713, is located in NBD1 directly adjacent to the phosphorylation sites of the R-domain and is coordinated by cation-pi interactions with two conserved arginine side chains, R765 and R1150 (Supplementary Fig

10.1007/BF00711382

It depends on the peptide and how you get it

Tail vein blood was drawn to measure plasma insulin with a mouse insulin ELISA kit (Novus) and plasma glucose using a One Touch Ultra glucometer (LifeScan)