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High content imaging allows the simultaneous screening of mitochondrial metabolism in a large number of samples using 96 well-plates or 384 well-plates

To assess the robustness of the findings, additional sensitivity analyses were performed using spline-based ANCOVA with cubic regression splines to accommodate potential non-linear relationships between age and metabolite abundance

Genetic factors, including deficiencies in enzymes for methionine synthase, cystathionine synthase, cystathionase, enzymes involved in folate metabolism, and proteins required for folate, vitamin B 6 or vitamin B 12 (e.g.,intrinsic factor) absorption can all contribute to hyperhomocysteinemia, and to an increased risk of cardiovascular disease