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doi: 10.1089/scd.2017.0069

Circulation 2010;122:S829-861

coli and yeast to more un-conventional systems such as Bacillus megaterium (Moore et al., 2018), Clostridium autoethanogenum (Krger et al., 2020), and eukaryote-derived systems such as rabbit reticulocyte lysate ( Initially, cell-free systems were prepared using cell lysates, where cells were lysed, chromosomal DNA and cell membrane debris were removed, and the rest of the cellular contents were used for studies