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The three ingredients with the strongest documented mechanisms for supporting melanin production in hair follicles are Bhringraj (which upregulates tyrosinase expression, the enzyme that synthesises melanin), Amla (which provides concentrated antioxidant protection to melanocytes and supports copper absorption for tyrosinase activity), and Mulethi (which contains glabridin, a compound that inhibits the enzyme responsible for melanin breakdown)

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doi: 10.1016/j.bbrc.2008.09.128

As discussed, a key limitation to the use of the CPZ model in vivo is the rapid remyelination seen on cessation of treatment (Blakemore, 1972)