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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

This gives us a much better idea of whether or not its needed, and how to correctly dose. What to Avoid in B12 Supplements King says there are certain things to look for and avoid when shopping for a B12 supplement