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Animals under anesthesia were then transferred to a nose cone and continued to receive 15% isoflurane at an O 2 set to 1.0 L/min and then exsanguinated by cardiac puncture using a 25 gauge needle followed by cervical dislocation

Forouzanfar MH, Liu P, Roth GA, Ng M, Biryukov S, Marczak L et al (2017) Global burden of hypertension and systolic blood pressure of at least 110 to 115 mm Hg, 19902015