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To define the microbiota structures that were affected by GTE and purified catechins, PLS-DA analysis was repeated using only the microbiota profiles in the HF+GTE, HF+EGCG, and HF+CAT groups (Component 1, R 2 =0.64, Q 2 =0.42

Briefly, all cell lines were maintained in Dulbeccos Modified Eagles Medium with glutaMAX (Gibco 31966-021) supplemented with 10% FBS (v/v) (Thermo Fisher A5256701)

These findings confirmed that GpoA, AhpD and TpxD play a relevant protective role during oxidative stress, potentially enabling the bacterium to persist in hostile environments encountered in different tissues during infection