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Transzonal projections (TZPs) analysis TZPs analysis was performed according to the previously reported methods [27], Briefly, COCs were cultured in maturation medium for different durations (9, 12, 15, 18, 21 h), fixed in 4% PFA for 30 min, and then incubated with PBS containing 0.5% TritonX-100 for 30 min, and transferred into PBS containing 1% BSA for additional 30 min

The Ca 2+ -dependent activator protein for secretion (CAPS1) is another priming factor during exocytosis of synaptic vesicles and granule exocytosis in neurons (Farina et al

Lee JH, Ha DH, Go HK, et al

doi: 10.4103/1673-5374.266060 317 KuwarOKKaliaN