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Notably, after 1 hour of incubation with tracer, no change in radioactivity was observed in any of the conditions ( Supplementary Figure S4C, S4D )

After this period, the cells were washed and sonicated in saline solution, centrifugated to obtain a clean solution, and measured in COBAS C311 autoanalyzer (Roche Diagnostics, IN, USA)

Pharmacol Ther 85:1128 Scordo MG, Spina E, Dahl ML, Gatti G, Perrucca E (2005) Influence of CYP2C9, 2C19 and 2D6 genetic polymorphisms on the steady-state plasma concentrations of the enantiomers of fluoxetine and norfluoxetine

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