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After changing culture medium to DMEM without serum, siRNAs (12.5 nM final concentration) were simultaneously transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Inc.) and incubated at 37C for 24 h

Compared with saline and free-drug controls, TBP-NP-treated mice demonstrated significantly improved range of motion and enhanced mechanical properties, confirming effective tendon-specific targeting and functional repair ( In vitro , PDGF-BB cmRNA enhanced tendon cell proliferation and migration, while IL1RA cmRNA reduced pro-inflammatory cytokine expression