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RNA-seq analysis Total RNA was extracted from the treated cells using TRIzol Reagent (Invitrogen, USA) according to the manufacturers protocol

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Lyophilized GHK-Cu should be stored at -20C and protected from light, as the copper complex can undergo photodegradation

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[DOI] [PubMed] [Google Scholar] 51.Vant Slot G., Humpf H.U

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Investigating Liver Detoxification and Methylation A targeted history and laboratory assessment may help distinguish excessive exposure from impaired methylation, elevated SAH, poor transsulfuration, low glutathione, biliary dysfunction or gut-derived toxic burden

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