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Site-directed mutagenesis, overexpression and isolation of the CACT proteins The previously constructed pMW7-WTratCACT recombinant plasmid containing the wild-type (WT) CACT cDNA was used to introduce the mutations in the CACT protein [12]

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Beyond the previously described cellular strategies and intercellular communication mechanisms, pharmacological and tissue engineering approaches are also being developed to promote cardiac regeneration