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This approach may help avoid long-term tolerance issues while maintaining results

10.1016/j.freeradbiomed.2020.05.006 71 WischhusenP.ParaillouxM.GeraertP

A consistent model goes with ligation leading to vessel injury, exposure of tissue factors, stasis, thrombosis, hemodynamic changes, arterial hypotension, and in particular, venous hypertension, which approximates values over 20 mm Hg in the inferior caval vein, thereby being roughly four times higher than those in normal rats

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