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Hepatocytes treated with 111 M for 4 hours experience an approximate 20% decrease in both cytoplasmic and mitochondrial GSH 39

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The concentration of 17 sICPs, including soluble form of B- and T- lymphocyte attenuator (sBTLA), sCD27, sCD28, T-cell immunoglobulin mucin-3 (sTIM-3), herpes virus entry mediator (sHVEM), sCD40, glucocorticoid-induced tumor necrosis factor receptor-related protein (sGITR), GITR ligand (sGITRL), lymphocyte-activation gene 3 (sLAG-3), sTLR-2, sPD-1, sCTLA-4, sCD80, sCD86, sPD-L1, sPD-L2, and inducible T-cell co-stimulator (sICOS) were measured using multiplexed fluorescent bead-based immunoassays with the Milliplex Map Kit (EMD Millipore Corporation, Burlington, MA, USA)