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Nonetheless, a primary challenge associated with vitrification cryopreservation is the oxidative stress induced by excessive ROS during the freezing and thawing processes, which adversely im-pacts cell function and viability (43)

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102 A Cochrane review concluded that flushing fallopian tubes with oil contrast media may improve the clinical pregnancy rate (OR 1.42, 95% CI: 1.101.85, 6 RCTs, 2598 women, I 2 = 41%, lowquality evidence), but with only three studies, there was not enough evidence to determine if the livebirth rate was affected

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