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In addition, it showed GSH-dependent high cytotoxicity on account of the rapid exhaust of intracellular GSH by both GSH depletion and biosynthesis inhibition

Replacement of Tyr110 in recombinant Y110A decreased both k cat app and 1/ values for the disulfide substrate and GSH in the GSSCys and HEDS assay (Supplementary Figs 18 and 19)