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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)

However, this method should only be done under medical supervision and is typically recommended in clinical settings rather than for routine self-use

Current therapeutic strategies primarily focus on the ZIP family of transporters, employing a variety of approaches, including antibody-drug conjugates (ADCs), siRNAs, and natural inhibitors (Table 1)