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Biomaterials delivery strategies to repair degenerated intervertebral discs by regulating the inflammatory microenvironment

PEG-it Virus Precipitation Solution (LV810A-1, System Biosciences) was added to the clarified supernatant at a 1:4 ratio (e.g., 3.5 mL PEG-it per 14 mL supernatant), mixed, and incubated overnight at 4 C on a rotator