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Performance of the method The modified GPx-DTNB method's performance was completed following the bioanalytical method validation guidelines set by the Committee for Medicinal Products for Human Use (Singh and Narsinghani 2023)

It consists of the tripeptide glycyl-L-histidyl-L-lysine complexed with a divalent copper ion, forming a high-affinity chelate that facilitates controlled copper delivery within biological systems

j , Mouse melanomas were pretreated with autophagy (3-MA), apoptosis (ZVAD), or necroptosis (GSK872) inhibitors then injected intravenously into C57BL mice and metastatic disease was assessed 1 month later by bioluminescence imaging