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Profiling of Plasma Metabolites Suggests Altered Mitochondrial Fuel Usage and Remodeling of Sphingolipid Metabolism in Individuals With Type 2 Diabetes and Kidney Disease

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3.4.1 Cardiac Dysfunction Energy metabolism suppression is one of the cornerstones of cardiac dysfunction in sepsis/endotoxemia

Biochemical analysis Rat lung tissues weighing about 200 mg each were homogenized using an ULTRA-TURRAX T-25 homogenizer (Janke & Kunkel, IKA Werke, Germany) using phosphate-buffered saline at a ratio of 1:9 (10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 2.7 mM KCl, 137 mM NaCl, pH 7.4)