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These items were formerly in the Category 1 list, and are now on the official positive list instead: Brilliant Blue G (also known as Coomassie Brilliant Blue G-250) Cantharidin (for topical use only) Diphenylcyclopropenone (for topical use only) N-acetyl-D-glucosamine (for topical use only) Squaric acid dibutyl ester (also known as dibutyl squarate, for topical use only) Thymol iodide (for topical use only) Four other items that were formerly on the Category 1 list have been removed, and the FDA has decided not to add them to the positive list: Oxitriptan (also known as 5-hydroxytryptophan) Piracetam Silver protein mild Tranilast You can find the final rule that established the positive list here, and the positive list itself is here

His159 serves as proton acceptor, which interacts directly with nitrogen of the peptide bond to be cleaved

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